How to correctly store interleukin ELISA kits after aliquoting

Activity protein Class aliquot Component retention

standard following aliquot, enzyme-labelled antibody, biotinylated antibody and other active protein . After sealing, store in a light-protected environment at -20°C; repeated freeze-thaw is strictly prohibited. Each time, remove only a single single-use portion of aliquot reagent, and use it in its entirety immediately after thawing.

Before freezing, clearly label the aliquot tubes with the component name, aliquot date and concentration details to avoid confusion between different batches of reagents.

Other aliquot Component preservation

Any unused portion after aliquot microplate items should be returned to their original aluminium foil pouches, with desiccant added. After sealing, store at -20°C. In high-humidity environments, place the pouches in an additional sealed food-storage container to prevent moisture from causing the antibody coating to become inactive.

The concentrated wash solution and specimen diluent prepared after step aliquot should be sealed and stored in a refrigerator at 2–8 °C. Should crystals form, these may be dissolved in a warm water bath; this will not affect subsequent use.

Storage restrictions

Under no circumstances should any reagents following aliquot be frozen directly to –80 °C, as this may cause irreversible damage to the protein structure.

Photosensitive reagents such as the TMB colouring solution following aliquot must be stored in sealed, brown containers away from light and refrigerated at 2–8 °C; exposure to light must be avoided to prevent oxidation and discolouration.

All aliquot reagents must be used within one month; after this period, their activity must be verified using a positive control, and they may only be used in experiments if they pass this test.

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