Scientific applications of 15 ml centrifuge tube

15ml centrifuge tube is a staple consumable in the fields of biopharmaceuticals, chemistry and scientific research. Thanks to its moderate capacity, high compatibility and safe, stable performance, it is suitable for the vast majority of routine experimental scenarios, ranging from cell culture to molecular testing.

Applications in Cell Biology Experiments

The 15 ml centrifuge tube is a frequently used consumable in cell-related procedures and is suitable for the vast majority of standard cell processing workflows:

Collection and concentration of cell suspensions: After pancreatic enzyme digestion of adherent cells, centrifuge the suspension directly at a low speed of 1000–1500 rpm centrifugation, replace culture medium or resuspend the cells to avoid the risk of contamination from repeated aliquot use of 1.5 ml tubes.

sterile System operation: DNase/RNase-free The pyrogen-free sterilized variant can be used directly in intermediate steps of cell passaging, primary cell isolation and immune cell culture, and is compatible with standard rotors for the vast majority of benchtop centrifugation machines.

Mild specimen Protection: U-shaped base centrifugation Cell sediment at the back is distributed in a thin layer and is not excessively compacted by the pointed base structure, significantly reducing mechanical damage during resuspension and better maintaining cell viability.

General-purpose vectors for molecular biology experiments

15ml centrifuge tube We undertake end-to-end processing for small and medium-sized specimen:

protein Purification and processing: Following on from protein crude extraction and antibody purification, separation of the supernatant from immunoprecipitation, buffer elution and other steps; some ultrafiltration units with a 15 ml capacity centrifuge tube can also directly achieve protein rapid concentration by a factor of 25–50.

enzyme Preparation of reaction mixtures: suitable for medium-scale PCR systems; enzyme preparation of premixes to facilitate reactions and brief incubation; the clear graduations on the tube body facilitate rapid volume adjustment.

Biology specimen Application scenarios

In testing and biological specimen library work, 15 ml centrifuge tube is the tool of choice for batch specimen pre-processing:

specimen Separation: Directly performs the separation of plasma and blood cells from blood specimen, serum separation, and the precipitation of impurities in urine, cerebrospinal fluid and other fluids specimen, suitable for standard clinical centrifugation conditions of 3000–5000 x g centrifugation.

specimen Short-term storage: PP material can withstand temperatures as low as -80°C; the markings on the walls of the frosted tubes will not become blurred or peel off even after repeated freeze-thaw, thus preventing specimen confusion.

Multiple batches specimen aliquot : Batch aliquot culture medium, buffer, test reagents; the scale error on the tube body is controlled within ±2 per cent, enabling rapid quantification without the need for an additional measuring cylinder.

Chemistry and general research support applications

In addition to biology experiments, it also meets a wide range of basic requirements for standard chemistry experiments:

Handling of corrosive reagents: High-purity PP material is resistant to corrosion by the vast majority of acids, alkalis and common organic solvents, and is used for the temporary storage, mixing and low-speed centrifugation purification of small to medium volumes of chemical reagents.

General-purpose specimen centrifugation Centrifugation: Used for the precipitation of impurities in environmental water samples, the separation of layers in soil leachates, and phase separation following the extraction of small-molecule compounds; suitable for standard centrifugation centrifugation forces of 7500xg or higher.

Universal compatibility with laboratory consumables: the standard outer diameter is compatible with rotors for almost all brands of benchtop centrifugation centrifuges, eliminating the need for additional adapters for specialised equipment and significantly reducing the cost of purchasing laboratory consumables.

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Note: The information on the scientific research products above is for reference only!